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Tuesday, May 5, 2020 | History

2 edition of Peptide derivatives for proteinase assays found in the catalog.

Peptide derivatives for proteinase assays

Janette Mary Sullivan

Peptide derivatives for proteinase assays

by Janette Mary Sullivan

  • 265 Want to read
  • 2 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, Dept of Chemistry.

Statementby Janette Mary Sullivan.
ID Numbers
Open LibraryOL13869916M

The Fifteen American Peptide Symposium (15APS) was held in Nashville, Tennessee, on June , This biennial meeting was jointly sponsored by the American Peptide Society and Vanderbilt University. The attendance of 1, participants from 37 countries was lower than the two previously held Symposia. However, the number of participating countries was the largest. Deubiquitinating Enzymes Inhibitors Fluorescent Peptide / Protein Substrates Ubiquitin Binding Proteins Ubiquitin / UBL Derivatives Apoptosis / Necrosis Antibodies Kits Protein Expression / Purification Reagents DNA / Protein Ladders.

To determine whether CDDO directly inhibits Lon, degradation assays were performed using purified protease and peptide or protein substrates. We used a fluorescently labeled reporter substrate (Z-Ala-Ala) 2 -Rh (AA 2 -Rh) consisting of nonnucleophilic alanine dipeptides flanking rhodamine , which cannot form adducts with by: The EnzChek Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to proteaseMissing: book.

A BODIPY Fluorescent Microplate Assay for Measuring Activity of Calpains and Other Proteases Valery F. Thompson,1 Sandra Saldan˜a, Jinyang Cong, and Darrel E. Goll Muscle Biology Group, University of Arizona, Tucson, Arizona Received Septem The use of File Size: KB. Combining NMR, mass spectrometry, AlphaLISA and cell assays, we discovered a compound C1 that binds C-terminal juxtamembrane lysines at the transmembrane domain of the amyloid precursor protein (APPTM) and inhibits γ-secretase production of amyloid-β with μM IC Our work suggests that targeting APPTM is a novel and viable strategy in AD drug discovery.


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Peptide derivatives for proteinase assays by Janette Mary Sullivan Download PDF EPUB FB2

Fluorescence intensity assays monitoring change from chemically quenched dyes. In this approach, a fluorescent dye that contains a reactive amine group can be covalently attached to the carboxyl end of a peptide substrate via an amide bond. The sequence of the peptide moiety provides protease specificity, and the dye moiety functions as a reporter for enzymatic : Guofeng Zhang.

Human adenovirus proteinase (AVP), the first member of a new class of cysteine proteinases, is required for the synthesis of infectious virus. As such, it is an attractive target for proteinase inhibitors that act as antiviral agents. However, before potential inhibitors can be screened, a quick, sensitive, and quantitative assay for the enzyme is by: 1.

In this context, several peptide derivatives containing electrophilic groups have been developed as potential inhibitors against this protease class, 56, 57, 58 Usually, electrophilic groups can be applied to the in vitro assays, but they are extremely reactive toward any nucleophile in vivo or advanced biological by: 4.

Fluorescent Derivatives of Diphenyl [1-(N-Peptidylamino)alkyl]phosphonate Esters: Synthesis and Use in the Inhibition and Cellular Localization of Serine Proteases.

Bioconjugate ChemistryCited by: The assays were performed with PbST enriched upon affinity chromatography in a p-aminobenzamidine (pABA)-Sepharose column. Although PbST can cleave the fluorescence resonance energy transfer peptide Abz-MKRLTL-EDDnp between L–T, the C(Npys) derivatives were not substrates nor were they toxic in a cell detachment assay, allowing therapeutic by: 3.

Title: The Determination and Use of Optimized Protease Substrates In Drug Discovery and Development VOLUME: 8 ISSUE: 28 Author(s):Paul L. Richardson Affiliation:Department 46Y, AP10, 2nd Floor, Abbott Laboratories, Abbott Park Rd., Abbott Park, IL Keywords:protease substrate library, protease assay, protease inhibitor, protease phage display, positional scanning library Cited by: The symposium consisted of 8 sessions with 42 oral and 90 poster presentations, including synthetic methods, molecular diversity and peptide libraries, structure and conformation of peptides and proteins, bioactive peptides, peptide immunology, De Novo design and synthesis of proteins and peptides, ligand-receptor interactions, the chemistry-biology-interface and challenging problems in peptides.

quantitative protease assay by substrate-agarose plate method Article (PDF Available) in Journal of microbiology, biotechnology and food sciences 6(2) October with 2, Reads. In one embodiment, the assay employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the by: 7.

4-TRIFLUOROMETHYLCOUMARIN PEPTIDE DERIVATIVES AND THEIR USE IN PROTEINASE ASSAYS (PAT - EP) Smith Robert Eugene Smith, Robert Eugene.

Patent: Publ. of which is an amino acid derivative of 7-aminotrifluoromethylcoumarin; Missing: book. Method for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-aminotrifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-aminotrifluoromethylcoumarin chromophore Cited by: 4-TRIFLUOROMETHYLCOUMARIN PEPTIDE DERIVATIVES AND THEIR USE IN PROTEINASE ASSAYS (PAT - WO) SMITH R, BISSELL E SMITH R.

Patent: the which is an amino acid derivative of 7-aminotrifluoromethylcoumarin; Missing: book. A bioluminescent general protease assay was developed using a combination of five luminogenic peptide substrates. The peptide-conjugated luciferin substrates were combined with luciferase to Cited by: 7.

A fluorous-based peptide microarray for protease screening was developed, and the comparison of a series of serine proteases showed that their ability to cleave peptide substrates in solution was. Title: Characterization and Inhibition of SARS-Coronavirus Main Protease VOLUME: 6 ISSUE: 4 Author(s):Po-Huang Liang Affiliation:Institute of BiologicalChemistry, Academia Sinica, TaipeiTaiwan R.O.C.

Keywords:SARS coronavirus, cysteine protease, fluorescence assay, high throughput screening, rational drug design, inhibitor Abstract: Severe acute respiratory syndrome (SARS) is an. The β-lactam ring represents a valuable moiety that can induce covalent binding of an inhibitor to its target.

In this study, we explored di- and tripeptides with β-lactam electrophilic warheads as inhibitors of dengue and West Nile virus NS2B-NS3 protease. Tripeptides with a (3S)-β-lactam moiety displayed the highest activity, with IC50 and EC50 values in the lower micromolar range in Author: Tonko Dražić, Sara Kopf, James Corridan, Mila M.

Leuthold, Branimir Bertoša, Christian D. Klein. Protocol Peptide ELISA Ready-to-use peptide ELISA Revision Contact us: THE Support: + Order per fax: + Or e-mail: [email protected] www: JPT Peptide Technologies GmbH Volmerstrasse 5 Berlin GERMANY Product Use & Liability SE PRODUCTS ARE FOR EXPERIMENTALFile Size: 97KB.

Among them, electrochemical methods, surface spectroscopy techniques, and enzyme-linked peptide protease assays are commonly used. Finally, recent developments in liquid crystal (LC)-based protease assays and their applications for detecting proteases and their inhibitors are discussed.

Cystatin C is a human cysteine proteinase inhibitor present in extracellular fluids 6. We have synthesized peptide derivatives mimicking the proposed proteinase-binding centre of cystatin C Cited by: Despite their many potential advantages, they have not been utilized in FRET-peptide substrates for protease assays.

Therefore, we evaluated two of the Alexa Fluor dyes, Alexa Fluor® and Alexa Fluor®in combination with QSY quenchers QSY®-7 and QSY® (Table 1), for their ability to serve as FRET-based, 3CLpro enzyme by:.

The latter methods employ chromogenic or fluorogenic peptide derivatives comprising a short amino acid sequence matching the preferred cleavage site of the protease as substrates.

As only one peptide bond is cleaved in each substrate, these assays permit kinetic by:   Fluorescent conjugated polyelectrolytes with pendant ionic sulfonate and carboxylate groups are used to sense protease activity.

Inclusion of the fluorescent conjugated polyelectrolyte into the assay scheme leads to amplification of the sensory response. The sensing mechanism relies on an electrostatic interaction between the conjugated polyelectrolyte and a peptide substrate that is labeled Cited by:   We describe here a simple assay that allows the visual detection of a protease.

The method takes advantage of the high molar absorptivity of the plasmon band of gold colloids and is based on the color change of their solution when treated with dithiols.

We used C- and N-terminal cysteinyl derivatives of a peptide substrate exploiting its selective recognition and cleavage by a specific by: